Ibogaine extraction from Iboga

[0utrider]

In this case it was for the root/ root bark of tabernanthe iboga used as the plant material, which may contain up to 2.5 % or 6 % alkaloids respectively.
The plant material was extracted with methanol four times, filtered and the methanol reduced to a small volume. An equal amount of water and acetic acid solution is added and shaken with petroleum naphtha, which is then separated and backwashed with acetic acid solution. All the aqueous phases are combined ( I gather that these processes remove non-alkaloidal components ).
The aqueous phases are reduced in volume, then basified with ammonia hydroxide. This is then extracted four times with ethylene dichloride ( possibly chloroform too ). The solvent is washed with water, dried and concentrated. An equal amount of ethanol is added and the whole reduced to the original volume, then about twice the amount of ethanol is added. After chilling in the fridge for two days or so, ibogaine crystallises out, and can be collected by filtration. The remaining liquid was again reduced in volume and re-chilled for a second crop of ibogaine.
Evaporation to dryness of the liquid yielded other alkaloids and residual ibogaine, which can be separated by chromatography, though can be laborious.
To purify the ibogaine 100 mg of the crude ibogaine, as obtained above, was dissolved in 1 l of acetone, then 53.1 ml (I don’t know why this much) of 1:1 HCl was added, with ibogaine HCl precipitating ( 108 mg in this case ) out straight away, this compound being relatively insoluble in acetone, compared to the base. Isolated by filtration.
ibogaine mp 151-153* C sol - ethanol, ether, chloroform, acetone
ibogaine HCl mp 299-300* C.
In tabernanthe iboga, ibogaine seems to be the most active and prominent alkaloid. In other species that are recorded as containing ibogaine, other alkaloids sometimes make up the majority of the alkaloids, with ibogaine being a minor component. Many related alkaloids however have a similar but not such strong action as ibogaine. The isolation of ibogaine from more complex mixtures of alkaloids may be a bit more tricky, especially if ibogaine is not a major component of the alkaloids.

ok this is certainly not a great manual, SWIM is really interested in that, does SWIY have maybe some ideas or manuals how to extract ibogaine?

[some_gurl]

I found this post/write-up on another forum.. may help..


EXPERIMENTAL SECTION

Extraction of T. iboga root (TA). One kg (2.5 L) of powdered T. iboga root and 5 L of 0.5% acetic acid were placed in a 6 L plastic bucket, stirred occasionally for one hour, and filtered through a cloth sack. The sack was wrung to expel all possible liquid from the root powder and the filtrate (pH = 3-4) was basified using 60 mL of 30% ammonia. The resulting flocculent, medium greenish-brown precipitate of TA was patiently gravity filtered through 30 cm filter paper and thoroughly rinsed with distilled water. This procedure was repeated twice more on the same root powder. The filter papers bearing the TA were placed on paper towels on a wire rack and left in a warm draft until successive weighings detected no more than 0.3% loss per day. The hard, dark brown solid weighed 30.037 g (3.0%) and was ground in a mortar and sifted to give a fine brown powder.

Conversion of alkaloids to the hydrochlorides (PTA HCl). 28.00 g of powdered TA was placed on a filter paper in a funnel and 450 mL of acetone was added in portions with gentle stirring. The funnel was removed and 2 mL of concentrated HCl was slowly added dropwise to the flask with swirling, occasionally adding a trace of PTA HCl from a previous batch to initiate precipitation. After waiting a few minutes to allow precipitation to begin, dropwise HCl (2.8 mL) was added with swirling until the liquid became acidic according to pH paper. A final 0.4 mL of HCl was added dropwise and the flask was placed in the refrigerator overnight. The yellow powder was scraped from the sides of the flask, filtered, rinsed with 84 mL of acetone, and dried at room temperature to give 9.493 g (33.9%) of PTA HCl. The black, spent TA weighed 14.521 g (51.9%) after drying.

Ibogaine HCl. 9.712 g of PTA HCl was patiently dissolved in 150 mL of boiling 95% ethanol, set overnight at room temperature, refrigerated for two hours, and the mother liquor was decanted from the yellow crystals (4.412 g). Recrystallizing again from 80 mL of 95% ethanol gave 3.666 g of mostly pure ibogaine HCl.

Recovery of residual alkaloids (RA). Most of the acetone was distilled from the filtrate from the preparation of PTA HCl and the remainder was evaporated using a stream of air. The dark residue was dissolved in 400 mL of distilled water, filtered, and basified to pH 9 using 3 mL of 30% ammonia. The medium yellow suspension was filtered through a fresh coffee filter paper and left on a warm surface to dry. The chunks of light, chalky, off-white alkaloid residue weighed 4.750 g (17.0%).

Extraction of V. africana trunk bark (VTA). One kg of powdered trunk bark was extracted in the same manner as the T. iboga root above, resulting in 59.723 g (6.0%) of crumbly brown voacanga total alkaloids (VTA).

Conversion of alkaloids to the hydrochlorides (VPTA HCl). 75.00 g of VTA was treated in a manner similar to the PTA HCl above, resulting in 35.929 g (43.6%) of medium brown VPTA HCl. The spent VTA weighed 31.534 g (42.0%).

[Motumba]

Grind the rootbark to a fine powder' then soak it in a volume of warm methanol' 60 degrees' do this 3-4 times until the methanol comes up a very pale light tan colour' first pull gets most of the alks and comes up the darkest'
Let settle' then decant'
Combine all of the methanol solutions and stick them in a bowl'
Stand the bowl in a pan of hot water and evaporate half of the methanol'
Add acetic acid to lower the ph to 3-4 ph nd keep cooking the bowl until it is completley dry'
The resultant dark resin can then be scraped up' store in a light proof airtight container'

Safe journeys'

Blessings

Motumba +

Reputation Comments on this post:

answers the question clearly

[0utrider]

did SWIY actually try this? can this be confirmed?

[Motumba]

Originally Posted by 0utrider

did SWIY actually try this? can this be confirmed?

Sorted ))

Be well'

Nobuoni +