Originally Posted by ronpintx
Given the striking geometric similarity between AM-2201
-- could the two be confused
in a urine test
Sure the molecular weight is different - but are false positives more likely in a case like this?
As far as I can tell, the metabolism of this would likely mirror JWH-018, ending with a nearly identical metabolite.
First, the biggest difference between these two molecules is the existence of the fluoropentyl indole side-chain (in AM-2201, which is absent in JWH-018). This is the first site of cleavage in napthoylindole metabolism, at least it seems to be the major trend in human metabolism. The molecular weight difference between these two molecules is entirely the result of the flourine atom, which will be removed with the rest of the pentyl side-chain (this, of course, is the site of debate for potential fluorotoxicity of fluoropentyl-indoles).
After this, the metabolic route continues to attack the indole ring, resulting in the detectable (via GC/MS or LC/MS-MS) indole-n-dealkyl metabolite, which as far as I can tell should look basically identical in either of the aforementioned methods.
Metabolic hydroxylation also occurs, but the metabolites of this route seem less extensive and are almost entirely conjugated with glucuronide.
READ: JWH-018 and AM-2201 will likely appear identical on basic laboratory drug-testing.
- This is entirely speculation, the fluoropentyl side-chain could alter metabolism in an unforeseen manner (unlikely, but worth a caveat).
- This is based on oral consumption
and degradation via first-pass metabolism. Pyrolytic byproducts may differ in their structure and retain activity, in theory. These products may not be metabolized via this mechanism. However, JWH-018 or AM-2201 which is systemically circulated will almost certainly follow the above scheme.